Cloning of AIP1, a novel protein that associates with the apoptosis-linked gene ALG-2 in a Ca2+-dependent reaction

J Biol Chem. 1999 Jan 15;274(3):1533-40. doi: 10.1074/jbc.274.3.1533.

Abstract

ALG-2 is a 22-kDa calcium-binding protein necessary for cell death induced by different stimuli in 3DO T-cell hybridoma. 3DO cell clones depleted of ALG-2 protein exhibit normal caspases activation, suggesting that ALG-2 function is required downstream or is independent of caspase proteases activity for apoptosis to occur. Using the yeast two-hybrid screening system, we have isolated and characterized the mouse cDNA encoding for ALG-2 interacting protein 1 (AIP1), a novel protein that interacts with ALG-2. ALG-2 and AIP1 colocalize in the cytosol and the presence of calcium is an indispensable requisite for their association. Sequence alignment shows that AIP1 is highly similar to BRO1, a yeast protein related to components of the Pkc1p-MAP kinase cascade. Overexpression of a truncated form of AIP1 protects two different cell types from death induced by trophic factors withdrawal; thus, our data indicate that AIP1 cooperates with ALG-2 in executing the calcium-dependent requirements along the cell death pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Apoptosis / genetics*
  • Apoptosis Regulatory Proteins
  • Blotting, Northern
  • COS Cells
  • Calcium / metabolism*
  • Calcium-Binding Proteins / genetics*
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cell Cycle Proteins
  • Cloning, Molecular
  • Cytosol / metabolism
  • DNA-Binding Proteins
  • Endosomal Sorting Complexes Required for Transport
  • Fungal Proteins / metabolism
  • HeLa Cells
  • Humans
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Rabbits
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors / metabolism

Substances

  • Apoptosis Regulatory Proteins
  • Calcium-Binding Proteins
  • Carrier Proteins
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Endosomal Sorting Complexes Required for Transport
  • Fungal Proteins
  • GAL4 protein, S cerevisiae
  • PDCD6 protein, human
  • PDCD6IP protein, human
  • Pdcd6 protein, mouse
  • Pdcd6ip protein, mouse
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Calcium

Associated data

  • GENBANK/AF119955