Core 2 oligosaccharide biosynthesis distinguishes between selectin ligands essential for leukocyte homing and inflammation

Immunity. 1998 Dec;9(6):881-90. doi: 10.1016/s1074-7613(00)80653-6.


Mammalian serine/threonine-linked oligosaccharides (O-glycans) are commonly synthesized with the Golgi enzyme core 2 beta-1,6-N-acetylglucosaminyltransferase (C2 GlcNAcT). Core 2 O-glycans have been hypothesized to be essential for mucin production and selectin ligand biosynthesis. We report that mice lacking C2 GlcNAcT exhibit a restricted phenotype with neutrophilia and a partial deficiency of selectin ligands. Loss of core 2 oligosaccharides reduces neutrophil rolling on substrata bearing E-, L-, and P-selectins and neutrophil recruitment to sites of inflammation. However, the diminished presence of L-selectin ligands on lymph node high endothelial venules does not affect lymphocyte homing. These studies indicate that core 2 oligosaccharide biosynthesis segregates the physiologic roles of selectins and reveal a function for the C2 GlcNAcT in myeloid homeostasis and inflammation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Movement*
  • Galactosyltransferases / genetics
  • Galactosyltransferases / metabolism
  • Galactosyltransferases / physiology*
  • Gene Targeting
  • L-Selectin / biosynthesis
  • Ligands
  • Mice
  • Mice, Inbred BALB C
  • Mutagenesis
  • Neutrophils / immunology
  • Neutrophils / physiology*
  • Oligosaccharides / metabolism*
  • Polysaccharides / metabolism


  • Ligands
  • Oligosaccharides
  • Polysaccharides
  • L-Selectin
  • Galactosyltransferases
  • glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase