Detection and sequence analysis of hepatitis B virus integration in peripheral blood mononuclear cells

J Virol. 1999 Feb;73(2):1235-8. doi: 10.1128/JVI.73.2.1235-1238.1999.

Abstract

A PCR-based technique was used to detect hepatitis B virus (HBV) integration in peripheral blood mononuclear cells from patients with chronic hepatitis B. Integrated HBV DNA sequences, with virus-cell junctions located in the cohesive region between direct repeat 1 (DR1) and DR2, were found in 2 of 10 studied patients.

MeSH terms

  • Base Sequence
  • DNA, Viral
  • Hepatitis B virus / genetics*
  • Hepatitis B, Chronic / virology*
  • Humans
  • Leukocytes, Mononuclear / virology*
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Sequence Analysis, DNA / methods*
  • Virus Integration*

Substances

  • DNA, Viral

Associated data

  • GENBANK/V00866