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. 1999 Feb;73(2):1695-8.

Nonstructural C Protein Is Required for Efficient Measles Virus Replication in Human Peripheral Blood Cells

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Free PMC article

Nonstructural C Protein Is Required for Efficient Measles Virus Replication in Human Peripheral Blood Cells

C Escoffier et al. J Virol. .
Free PMC article

Abstract

The P gene of measles virus (MV) encodes the phosphoprotein, a component of the virus ribonucleoprotein complex, and two nonstructural proteins, C and V, with unknown functions. Growth of recombinant MV, defective in C or V expression, was explored in human peripheral blood mononuclear cells (PBMC). The production of infectious recombinant MV V- was comparable to that of parental MV tag in simian Vero fibroblasts and in PBMC. In contrast, MV C- progeny was strongly reduced in PBMC but not in Vero cells. Consistently, the expression of both hemagglutinin and fusion proteins, as well as that of nucleoprotein mRNA, was lower in MV C--infected PBMC. Thus, efficient replication of MV in natural host cells requires the expression of the nonstructural C protein. The immunosuppression that accompanies MV infection is associated with a decrease in the in vitro lymphoproliferative response to mitogens. MV C- was as potent as MV tag or MV V- in inhibiting the phytohemagglutinin-induced proliferation of PBMC, indicating that neither the C protein nor the V protein is directly involved in this effect.

Figures

FIG. 1
FIG. 1
Kinetics of infectious virus production by human PBMC and Vero cells infected with MV tag (diamonds), MV C (circles), or MV V (triangles). The activated PBMC and Vero cells were infected at MOIs of 1 and 0.01, respectively.
FIG. 2
FIG. 2
Cell surface expression of the H protein after infection of human PBMC and Vero cells with MV tag, MV C, or MV V at MOIs of 1 and 0.01, respectively. (A) Histogram profile of H expression 2 days after mock infection (solid histograms) or MV infection (open histograms). (B) Kinetics of H expression after infection of PBMC with no virus (open squares), MV tag (solid diamonds), MV C (solid circles), or MV V (solid triangles).
FIG. 3
FIG. 3
Western blot analysis of the expression of H and F proteins by human PBMC infected with MV tag, MV C, or MV V at an MOI of 1 at day 3 p.i. S6K, S6 kinase.
FIG. 4
FIG. 4
MV-induced inhibition of PBMC proliferation. PBMC were stimulated with PHA and interleukin 2 and infected with the indicated virus at an MOI of 1. The proliferation level was determined by [3H]thymidine incorporation, and results are expressed as counts per minute (means of triplicates ± standard deviations).

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