Wavelength specific activation of PI 3-kinase by UVB irradiation

FEBS Lett. 1998 Dec 18;441(2):297-301. doi: 10.1016/s0014-5793(98)01565-8.


We investigated the effect of UVB irradiation on phospholipid metabolism using normal human diploid skin fibroblasts. When cells were exposed to monochromatic UV light, the intracellular PIP3 level was increased within 1 min in a wavelength-specific manner. The increase was most marked in the UVB range and was inhibited by a PI 3-kinase inhibitor, tyrosine kinase inhibitor, or antioxidant reagents. Furthermore, the decrease of cell viability induced by UVB irradiation was significantly blocked by wortmannin. These results suggest that PI 3-kinase activation is involved in novel cellular responses specific for UVB that lead to cell death.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Androstadienes / pharmacology
  • Cell Survival / radiation effects
  • Cells, Cultured
  • Enzyme Activation / radiation effects
  • Enzyme Inhibitors / pharmacology
  • Humans
  • Phosphatidylinositol 3-Kinases / metabolism*
  • Phosphoinositide-3 Kinase Inhibitors
  • Protein-Tyrosine Kinases / metabolism
  • Reactive Oxygen Species
  • Ultraviolet Rays*
  • Wortmannin


  • Androstadienes
  • Enzyme Inhibitors
  • Phosphoinositide-3 Kinase Inhibitors
  • Reactive Oxygen Species
  • Protein-Tyrosine Kinases
  • Wortmannin