The phosphatase Cdc14 triggers mitotic exit by reversal of Cdk-dependent phosphorylation

Mol Cell. 1998 Dec;2(6):709-18. doi: 10.1016/s1097-2765(00)80286-5.

Abstract

Exit from mitosis requires the inactivation of mitotic cyclin-dependent kinases (CDKs) by an unknown mechanism. We show that the Cdc14 phosphatase triggers mitotic exit by three parallel mechanisms, each of which inhibits Cdk activity. Cdc14 dephosphorylates Sic1, a Cdk inhibitor, and Swi5, a transcription factor for SIC1, and induces degradation of mitotic cyclins, likely by dephosphorylating the activator of mitotic cyclin degradation, Cdh1/Hct1. Feedback between these pathways may lead to precipitous collapse of mitotic CDK activity and help coordinate exit from mitosis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anaphase-Promoting Complex-Cyclosome
  • Cdh1 Proteins
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / physiology*
  • Cyclin B*
  • Cyclin-Dependent Kinase Inhibitor Proteins
  • Cyclin-Dependent Kinases / metabolism*
  • Cyclins / metabolism
  • DNA Replication / genetics
  • DNA-Binding Proteins*
  • Fungal Proteins / metabolism
  • Fungal Proteins / physiology
  • GTP-Binding Proteins
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Fungal
  • Ligases / metabolism
  • Ligases / physiology
  • Mitosis / physiology*
  • Mutation
  • Phosphoprotein Phosphatases / genetics
  • Phosphoprotein Phosphatases / physiology
  • Phosphorylation
  • Protein Kinases / physiology
  • Protein Tyrosine Phosphatases*
  • Protein-Serine-Threonine Kinases
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins / genetics
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins*
  • Substrate Specificity
  • Transcription Factors / metabolism
  • Transcription, Genetic
  • Ubiquitin-Protein Ligase Complexes*
  • Ubiquitin-Protein Ligases

Substances

  • CDC14 protein, S cerevisiae
  • CDC15 protein
  • CDH1 protein, S cerevisiae
  • CEF1 protein, S cerevisiae
  • CLB2 protein, S cerevisiae
  • Cdh1 Proteins
  • Cell Cycle Proteins
  • Cyclin B
  • Cyclin-Dependent Kinase Inhibitor Proteins
  • Cyclins
  • DNA-Binding Proteins
  • Fungal Proteins
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins
  • SIC1 protein, S cerevisiae
  • SWI5 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Ubiquitin-Protein Ligase Complexes
  • Anaphase-Promoting Complex-Cyclosome
  • Ubiquitin-Protein Ligases
  • Protein Kinases
  • DBF2 protein, S cerevisiae
  • Protein-Serine-Threonine Kinases
  • Cyclin-Dependent Kinases
  • Phosphoprotein Phosphatases
  • Protein Tyrosine Phosphatases
  • GTP-Binding Proteins
  • Ligases