A novel transcriptional coactivator, p52, functionally interacts with the essential splicing factor ASF/SF2

Mol Cell. 1998 Dec;2(6):751-9. doi: 10.1016/s1097-2765(00)80290-7.


Increasing evidence suggests that pre-mRNA splicing can take place cotranscriptionally in vivo. However, insight into how these two processes are linked has been lacking. Here, we describe that a novel transcriptional coactivator, p52, interacts not only with transcriptional activators and general transcription factors to enhance activated transcription but also with the essential splicing factor ASF/SF2 both in vitro and in vivo to modulate ASF/SF2-mediated pre-mRNA splicing. Furthermore, immunofluorescence studies indicate that the majority of endogenous p52 is colocalized with ASF/SF2 in the nucleoplasm of HeLa cells. Together, these observations suggest that, in addition to functioning as a transcriptional coactivator, p52 may also act as an adaptor to coordinate pre-mRNA splicing and transcriptional activation of class II genes.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Cell-Free System
  • HeLa Cells
  • Humans
  • Microscopy, Confocal
  • Nuclear Proteins / metabolism*
  • RNA Precursors / genetics
  • RNA Precursors / metabolism
  • RNA Splicing*
  • RNA-Binding Proteins
  • Serine-Arginine Splicing Factors
  • Sp1 Transcription Factor / pharmacology
  • Trans-Activators / metabolism*
  • Trans-Activators / pharmacology
  • Transcription Factors
  • Transcription, Genetic / drug effects
  • Transcriptional Activation / drug effects


  • Adaptor Proteins, Signal Transducing
  • Nuclear Proteins
  • PSIP1 protein, human
  • RNA Precursors
  • RNA-Binding Proteins
  • Sp1 Transcription Factor
  • Trans-Activators
  • Transcription Factors
  • Serine-Arginine Splicing Factors