The ATM-related cofactor Tra1 is a component of the purified SAGA complex

Mol Cell. 1998 Dec;2(6):863-7. doi: 10.1016/s1097-2765(00)80300-7.

Abstract

The SAGA histone acetyltransferase/transcriptional adaptor complex is composed of multiple transcriptional regulators including Ada, Spt, and TAFII proteins. Here we identify an additional novel subunit of the complex, Tra1, an ATM/PI-3-kinase-related homolog of the human TRRAP cofactor, which is essential for c-Myc and E2F-mediated oncogenic transformation. Mass spectrometry, immunoblotting, and immunoprecipitation experiments confirm the stable association of this protein within SAGA. In addition, the Tra1 protein is a component of at least two other histone acetyltransferase protein complexes. These results indicate a role for Tra1 in the regulation of transcriptional activation through the recruitment of HAT activity to an activator-bound promoter.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetyltransferases / metabolism*
  • Amino Acid Sequence
  • Ataxia Telangiectasia Mutated Proteins
  • Cell Cycle Proteins
  • DNA-Binding Proteins*
  • Electrophoresis, Polyacrylamide Gel
  • Fungal Proteins / physiology
  • Histone Acetyltransferases
  • Immunoblotting
  • Molecular Sequence Data
  • Multienzyme Complexes / chemistry
  • Multienzyme Complexes / isolation & purification
  • Multienzyme Complexes / metabolism
  • Protein Kinases / physiology
  • Protein-Serine-Threonine Kinases*
  • Proteins / analysis*
  • Proteins / metabolism*
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae Proteins*
  • Tumor Suppressor Proteins

Substances

  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Fungal Proteins
  • Multienzyme Complexes
  • Proteins
  • Saccharomyces cerevisiae Proteins
  • TRA1 protein, S cerevisiae
  • Tumor Suppressor Proteins
  • Acetyltransferases
  • Histone Acetyltransferases
  • Protein Kinases
  • ATM protein, human
  • Ataxia Telangiectasia Mutated Proteins
  • Protein-Serine-Threonine Kinases