The 400 kDa subunit of the PCAF histone acetylase complex belongs to the ATM superfamily

Mol Cell. 1998 Dec;2(6):869-75. doi: 10.1016/s1097-2765(00)80301-9.


PCAF histone acetylase is found in a complex with more than 20 associated polypeptides. Here we report cloning and characterization of the 400 kDa PCAF-associated factor referred to as PAF400. PAF400 is almost identical to TRRAP, which binds to c-Myc and E2F, and has significant sequence similarities to the ATM superfamily including FRAP, ATM, ATR, and the catalytic subunit of DNA-PK. Remarkably, PAF400 and FRAP share sequence similarity in broad regions that cover 80% of the entire PAF400 sequence. However, unlike the other members of the ATM superfamily, PAF400 is not a protein kinase as judged from the lack of kinase motif and autophosphorylation activity. We discuss the possibility that PAF400 may play a role in signaling of DNA damage to p53 by stimulation of p53 acetylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyltransferases / genetics*
  • Acetyltransferases / metabolism
  • Amino Acid Sequence
  • Ataxia Telangiectasia Mutated Proteins
  • Base Sequence
  • Cell Cycle Proteins
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • DNA-Binding Proteins
  • HeLa Cells
  • Histone Acetyltransferases
  • Humans
  • Molecular Sequence Data
  • Phosphorylation
  • Protein-Serine-Threonine Kinases*
  • Proteins / genetics*
  • Proteins / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tumor Suppressor Proteins


  • Cell Cycle Proteins
  • DNA, Complementary
  • DNA-Binding Proteins
  • Proteins
  • Saccharomyces cerevisiae Proteins
  • Tumor Suppressor Proteins
  • Acetyltransferases
  • Histone Acetyltransferases
  • ATM protein, human
  • Ataxia Telangiectasia Mutated Proteins
  • Protein-Serine-Threonine Kinases

Associated data

  • GENBANK/AF110377