3,5,3'-Triiodothyronine actively stimulates UCP in brown fat under minimal sympathetic activity

Am J Physiol. 1999 Jan;276(1):E179-87. doi: 10.1152/ajpendo.1999.276.1.E179.

Abstract

To investigate the role of type II 5'-deiodinase (5'D-II) in the expression of mitochondrial uncoupling protein (UCP) in brown adipose tissue (BAT), we injected intact male rats with reverse (r) 3,5,3'-triiodothyronine (T3; 100 micrograms. 100 g body wt-1. day-1), an inhibitor of 5'D-II, for 2-5 days. UCP decreased by approximately 20% in rats kept at 28 degreesC and failed to increase during cold exposure (4 degreesC). Next, thyroxine treatment (1-10 micrograms. 100 g body wt-1. day-1) increased nuclear T3 in rats kept at 28 or 4 degreesC. In these rats, nuclear T3 correlated positively with UCP. In addition, T3 (1-50 micrograms. 100 g body wt-1. day-1) given to intact rats (5-15 days; 28 degreesC) induced an approximately twofold increase in UCP. In these T3-treated animals, the interscapular BAT thermal response to norepinephrine infusion also correlated positively with T3 dose and UCP content. Treatment with propranolol or reserpine failed to block the T3 induction of UCP (approximately 1.8- and approximately 2.3-fold). The results emphasize the importance of local 5'D-II and reveal an independent role of T3 in the expression of UCP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue, Brown / drug effects*
  • Adipose Tissue, Brown / innervation
  • Adipose Tissue, Brown / metabolism*
  • Adipose Tissue, Brown / physiology
  • Adrenergic Antagonists / pharmacology
  • Animals
  • Body Temperature Regulation / drug effects
  • Body Temperature Regulation / physiology
  • Carrier Proteins / metabolism*
  • Cell Nucleus / metabolism
  • Cold Temperature
  • Enzyme Inhibitors / pharmacology
  • Iodide Peroxidase / antagonists & inhibitors
  • Ion Channels
  • Isoenzymes / antagonists & inhibitors
  • Male
  • Membrane Proteins / metabolism*
  • Mitochondrial Proteins
  • Norepinephrine / pharmacology
  • Rats
  • Rats, Wistar
  • Receptors, Thyroid Hormone / metabolism
  • Sympathetic Nervous System / physiology*
  • Thyroxine / pharmacology
  • Triiodothyronine / metabolism
  • Triiodothyronine / pharmacology*
  • Triiodothyronine, Reverse / pharmacology
  • Uncoupling Protein 1

Substances

  • Adrenergic Antagonists
  • Carrier Proteins
  • Enzyme Inhibitors
  • Ion Channels
  • Isoenzymes
  • Membrane Proteins
  • Mitochondrial Proteins
  • Receptors, Thyroid Hormone
  • Uncoupling Protein 1
  • Triiodothyronine
  • Triiodothyronine, Reverse
  • Iodide Peroxidase
  • Thyroxine
  • Norepinephrine