Gorilla RH-like genes and antigens

Immunogenetics. 1999 Feb;49(2):125-33. doi: 10.1007/s002510050471.


It has been previously shown that most of the human IgG monoclonal D-specific antibodies define a polymorphism in the gorilla consisting of two phenotypes: Dgor-positive and Dgor-negative. By quantitative indirect immunofluorescence assay and quantitative immunoblotting it was evaluated that the number of Dgor antigenic sites per gorilla red cell varies from a level equivalent to that observed for human RhD-positive cells to a level eight times higher. By immunoblotting with a rabbit reagent specific for the carboxylic end of human Rh-polypeptides it was demonstrated that RBCs from all gorillas, whatever their Dgor phenotype, possess 33000 relative molecular mass Rh-like polypeptides. The expression of the Dgor antigen was shown to be associated with the presence of three polymorphic bands defined by Southern blot using a human exon 4 RHCE probe, and to a length polymorphism of gorilla intron 3 evidenced by polymerase chain reaction. By contrast, the expression of the Dgor antigen was not associated to the length polymorphism of gorilla intron 4 which is related to the presence or absence of an Alu-Sx element in intron 4, paralleling the situation observed in human. These results confirmed the presence in the gorilla genome of at least two RH-like genes, one of which being responsible for Dgor polymorphism. The phylogenesis of the human and gorilla RH genes is discussed in light of the comparison of intron 4 sequences.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / blood
  • Antigen-Antibody Reactions
  • Base Sequence
  • Blotting, Southern
  • Exons
  • Glycoproteins / blood
  • Glycoproteins / genetics*
  • Gorilla gorilla / blood
  • Gorilla gorilla / genetics*
  • Humans
  • Immunoblotting
  • Introns
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Rh-Hr Blood-Group System / blood
  • Rh-Hr Blood-Group System / genetics*


  • Antibodies, Monoclonal
  • Glycoproteins
  • RHCE protein, human
  • Rh-Hr Blood-Group System
  • Rho(D) antigen

Associated data

  • GENBANK/AF049913
  • GENBANK/AF050636
  • GENBANK/AF071197