Trypan blue capsule staining to visualize the capsulorhexis in cataract surgery

J Cataract Refract Surg. 1999 Jan;25(1):7-9. doi: 10.1016/s0886-3350(99)80004-2.


A capsulorhexis may be difficult to perform in the absence of a red fundus reflex. Using 0.1 mL of trypan blue 0.1% to stain the anterior capsule in 30 patients with a mature cataract enabled us to visualize the capsulorhexis during phacoemulsification. No adverse reactions were observed up to 12 months after surgery. Trypan blue staining of the anterior capsule appears to be a safe technique to facilitate the performance of a capsulorhexis in the absence of a red fundus reflex.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsulorhexis / methods*
  • Humans
  • Lens Capsule, Crystalline / surgery*
  • Phacoemulsification
  • Safety
  • Staining and Labeling / methods*
  • Trypan Blue*


  • Trypan Blue