Reduced pathogenicity of a Candida albicans MAP kinase phosphatase (CPP1) mutant in the murine mastitis model

APMIS. 1998 Nov;106(11):1049-55. doi: 10.1111/j.1699-0463.1998.tb00257.x.

Abstract

Candida albicans strains with a deletion of the mitogen-activated protein kinase tyrosine phosphatase gene (CPP1) are derepressed in the yeast-to-hyphal transition on solid surfaces in vitro at ambient temperatures and this gene is therefore required for repression of the yeast-to-hyphal switch. The pathology caused by a CPP1 null mutant strain was compared with that of the null mutant into which the wild-type CPP1 gene was introduced by homologous recombination and with the wild-type parent strain in a murine mycotic mastitis model. The mammary glands of lactating mice (at day 5 postpartum) were infected for 2, 4 and 6 days with 1 x 10(5), 1 x 10(6) and 1 x 10(7) cell-forming units before euthanasia. Infected and non-infected control glands were evaluated histopathologically. The null mutant strains showed less severe pathology than the two control strains. The Cpplp tyrosine phosphatase may thus be considered a virulence determinant during localized infection in C. albicans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / genetics
  • Candida albicans / genetics
  • Candida albicans / pathogenicity*
  • Female
  • Male
  • Mastitis / microbiology*
  • Mice
  • Mice, Inbred BALB C
  • Mutation
  • Protein Tyrosine Phosphatases / genetics*
  • Virulence / genetics

Substances

  • Bacterial Proteins
  • Protein Tyrosine Phosphatases