The mechanism of action of T7 DNA polymerase

Curr Opin Struct Biol. 1998 Dec;8(6):704-12. doi: 10.1016/s0959-440x(98)80089-4.


The recent crystal structure determination of T7 DNA polymerase complexed to a deoxynucleoside triphosphate and primer-template DNA has provided the first glimpse of a replicative DNA polymerase in a catalytic complex. The structure complements many functional and structural studies of this and other DNA polymerases, allowing a detailed evaluation of proposals for the mechanism of nucleotidyl transfer and the exploration of the basis for the high fidelity of template-directed DNA synthesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Binding Sites
  • DNA-Directed DNA Polymerase / chemistry
  • DNA-Directed DNA Polymerase / metabolism*
  • Deoxyribonucleotides / chemistry
  • Hydrogen Bonding
  • Metals / chemistry
  • Protein Conformation


  • Deoxyribonucleotides
  • Metals
  • bacteriophage T7 induced DNA polymerase
  • DNA-Directed DNA Polymerase