The MHC of a broiler chicken line: serology, B-G genotypes, and B-F/B-LB sequences

Immunogenetics. 1999 Mar;49(3):215-24. doi: 10.1007/s002510050482.


Although the major histocompatibility complex of chickens (encoded in the B complex) has been studied for a number of years, almost all work has focused on the White Leghorn breed. Broiler (meat-type) chickens were derived from other breeds, including Cornish and Plymouth Rock. It was our hypothesis that new B haplotypes, not previously identified in White Leghorns, might be present in lines of broiler chickens. Furthermore, alloantisera used to identify B serotypes in Leghorn lines reportedly do not work well outside the line in which they were raised, with the result that broiler B haplotypes have not been incorporated into the universal nomenclature system. Our approach was to use a panel of B alloantisera produced to identify B serotypes within a commercial broiler breeder line (designated line A). B homozygotes identified serologically were compared by B-G genotyping using restriction fragment length polymorphism analysis. Furthermore, reverse transcription-polymerase chain reaction was used to amplify variable domains of expressed B-LB and B-F genes of homozygotes of most of the B serotypes in Line A, followed by cloning and nucleotide sequence determination. Comparison of B-LB and B-F sequences with standard Leghorn haplotypes demonstrated the existence of new alleles of B-L and B-F in a broiler breeder line, as well as the presence of alleles previously identified in Leghorns. In some cases, Leghorn-type alleles were in linkage with different B-G alleles in the broiler line than the common haplotypic associations found in Leghorn lines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Base Sequence
  • Chickens / blood
  • Chickens / classification*
  • Chickens / genetics*
  • Chickens / immunology
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Exons / genetics
  • Genes, MHC Class I / genetics
  • Genes, MHC Class II / genetics
  • Genotype
  • Haplotypes / genetics
  • Immune Sera
  • Major Histocompatibility Complex / genetics*
  • Molecular Sequence Data
  • Polymorphism, Restriction Fragment Length
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Serotyping*


  • DNA, Complementary
  • Immune Sera

Associated data

  • GENBANK/AF026560
  • GENBANK/AF026561
  • GENBANK/AF026562
  • GENBANK/AF026913
  • GENBANK/AF026914
  • GENBANK/AF026915
  • GENBANK/AF026916