Expression profiling using cDNA microarrays

Nat Genet. 1999 Jan;21(1 Suppl):10-4. doi: 10.1038/4434.


cDNA microarrays are capable of profiling gene expression patterns of tens of thousands of genes in a single experiment. DNA targets, in the form of 3' expressed sequence tags (ESTs), are arrayed onto glass slides (or membranes) and probed with fluorescent- or radioactively-labelled cDNAs. Here, we review technical aspects of cDNA microarrays, including the general principles, fabrication of the arrays, target labelling, image analysis and data extraction, management and mining.

Publication types

  • Review

MeSH terms

  • Animals
  • DNA, Complementary / chemical synthesis
  • DNA, Complementary / genetics*
  • Databases, Factual
  • Expressed Sequence Tags
  • Gene Expression*
  • Humans
  • Image Processing, Computer-Assisted
  • Molecular Probe Techniques*
  • Oligonucleotide Array Sequence Analysis / methods*


  • DNA, Complementary