Making and reading microarrays

Nat Genet. 1999 Jan;21(1 Suppl):15-9. doi: 10.1038/4439.


There are a variety of options for making microarrays and obtaining microarray data. Here, we describe the building and use of two microarray facilities in academic settings. In addition to specifying technical detail, we comment on the advantages and disadvantages of components and approaches, and provide a protocol for hybridization. The fact that we are now making and using microarrays to answer biological questions demonstrates that the technology can be implemented in a university environment.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • DNA / chemistry
  • DNA / metabolism
  • Glass
  • Lasers
  • Oligonucleotide Array Sequence Analysis / instrumentation*
  • Oligonucleotide Array Sequence Analysis / methods*
  • Robotics / instrumentation
  • Robotics / methods


  • DNA