Prader-Willi syndrome is caused by disruption of the SNRPN gene

Am J Hum Genet. 1999 Jan;64(1):70-6. doi: 10.1086/302177.

Abstract

A Prader-Willi syndrome patient is described who has a de novo balanced translocation, (4;15)(q27;q11.2)pat, with breakpoints lying between SNRPN exons 2 and 3. Parental-origin studies indicate that there is no uniparental disomy and no apparent deletion. This patient expresses ZNF127, SNRPN exons 1 and 2, IPW, and D15S227E (PAR1) but does not express either SNRPN exons 3 and 4 or D15S226E (PAR5), as assayed by reverse transcription-PCR, of peripheral blood cells. Methylation studies showed normal biparental patterns of inheritance of loci DN34/ZNF127, D15S63, and SNRPN exon 1. Results for this patient and that reported by Sun et al. support the contention that an intact genomic region and/or transcription of SNRPN exons 2 and 3 play a pivotal role in the manifestations of the major clinical phenotype in Prader-Willi syndrome.

Publication types

  • Case Reports
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • African Continental Ancestry Group / genetics
  • Autoantigens / genetics*
  • Blotting, Southern
  • Chromosome Banding
  • Chromosomes, Human, Pair 15*
  • Chromosomes, Human, Pair 4*
  • Exons
  • Humans
  • In Situ Hybridization, Fluorescence
  • Male
  • Polymerase Chain Reaction
  • Prader-Willi Syndrome / genetics*
  • Ribonucleoproteins, Small Nuclear / genetics*
  • Translocation, Genetic
  • snRNP Core Proteins

Substances

  • Autoantigens
  • Ribonucleoproteins, Small Nuclear
  • SNRPN protein, human
  • snRNP Core Proteins