Restricted high level expression of Tcf-4 protein in intestinal and mammary gland epithelium

Am J Pathol. 1999 Jan;154(1):29-35. doi: 10.1016/S0002-9440(10)65247-9.


Tcf-4 is a member of the Tcf/Lef family of transcription factors that interact functionally with beta-catenin to mediate Wnt signaling in vertebrates. We have previously demonstrated that the tumor suppressor function of APC in the small intestine is mediated via regulation of Tcf-4/beta-catenin transcriptional activity. To gain further insight into the role of Tcf-4 in development and carcinogenesis we have generated several mouse monoclonal antibodies, one of which is specific for Tcf-4 and another of which recognizes both Tcf-3 and Tcf-4. Immunohistochemistry performed with the Tcf 4- specific monoclonal antibody revealed high levels of expression in normal intestinal and mammary epithelium and carcinomas derived therefrom. Additional sites of Tcf-3 expression, as revealed by staining with the Tcf-3/-4 antibody, occurred only within the stomach epithelium, hair follicles, and keratinocytes of the skin. A temporal Tcf-4 expression gradient was observed along the crypt-villus axis of human small intestinal epithelium: strong Tcf-4 expression was present within the crypts of early (week 16) human fetal small intestine, with the villi showing barely detectable Tcf-4 protein levels. Tcf-4 expression levels increased dramatically on the villi of more highly developed (week 22) fetal small intestine. We conclude that Tcf-4 exhibits a highly restricted expression pattern related to the developmental stage of the intestinal epithelium. The high levels of Tcf-4 expression in mammary epithelium and mammary carcinomas may also indicate a role in the development of this tissue and breast carcinoma.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Breast / metabolism*
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology
  • COS Cells
  • Carcinoma / metabolism
  • Carcinoma / pathology
  • Cell Nucleus / metabolism
  • Colonic Neoplasms / metabolism
  • Colonic Neoplasms / pathology
  • Cytoskeletal Proteins / metabolism
  • Epithelium / metabolism
  • Female
  • Fetus
  • HMGB Proteins*
  • Humans
  • Immunohistochemistry / methods
  • Intestinal Mucosa / metabolism*
  • Intestines / embryology
  • Mice
  • Mice, Inbred BALB C
  • Staining and Labeling
  • TCF Transcription Factors
  • Trans-Activators*
  • Transcription Factor 7-Like 1 Protein
  • Transcription Factor 7-Like 2 Protein
  • Transcription Factors / metabolism*
  • beta Catenin


  • Antibodies, Monoclonal
  • CTNNB1 protein, human
  • CTNNB1 protein, mouse
  • Cytoskeletal Proteins
  • HMGB Proteins
  • TCF Transcription Factors
  • TCF7L1 protein, human
  • TCF7L2 protein, human
  • Tcf7l1 protein, mouse
  • Tcf7l2 protein, mouse
  • Trans-Activators
  • Transcription Factor 7-Like 1 Protein
  • Transcription Factor 7-Like 2 Protein
  • Transcription Factors
  • beta Catenin