Interleukin 8 (IL-8) induces the expression of kinin B1 receptor in human lung fibroblasts

Biochem Biophys Res Commun. 1998 Dec 30;253(3):750-5. doi: 10.1006/bbrc.1998.9848.


Kinin B1 receptors are induced by various inflammatory mediators. The aim of the present study was to investigate the effect of the CXC chemokine IL-8 on kinin B1 receptor expression in IMR-90 cells, by performing binding studies and Northern blot analysis of B1 receptor mRNA levels. We demonstrated here that the density of the kinin B1 receptors could be increased by the chemokine IL-8 in a concentration- and time-dependent manner. IL-8 also increased the kinin B1 receptor mRNA level in IMR-90 cells. IL-8-induced B1 receptor expression could be totally abolished by pretreatment with the metabolic inhibitors. Furthermore, expression was markedly reduced by antibodies to human IL-1alpha. In conclusion, IL-8 increased the expression of kinin B1 receptors in IMR-90 cells and this effect is likely to be secondary to the production of IL-1beta.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / pharmacology
  • Antigens, CD / biosynthesis
  • Antigens, CD / genetics
  • Cell Line
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Humans
  • Interleukin-1 / immunology
  • Interleukin-8 / immunology
  • Interleukin-8 / pharmacology*
  • Kallidin / analogs & derivatives
  • Kallidin / metabolism
  • Lung / cytology
  • Lung / drug effects*
  • Lung / embryology
  • Protein Binding
  • RNA, Messenger / analysis
  • Receptor, Bradykinin B1
  • Receptors, Bradykinin / biosynthesis*
  • Receptors, Bradykinin / genetics
  • Receptors, Interleukin / biosynthesis
  • Receptors, Interleukin / genetics
  • Receptors, Interleukin-8A


  • Antibodies
  • Antigens, CD
  • Interleukin-1
  • Interleukin-8
  • RNA, Messenger
  • Receptor, Bradykinin B1
  • Receptors, Bradykinin
  • Receptors, Interleukin
  • Receptors, Interleukin-8A
  • bradykinin, Lys-Leu(8)-desArg(9)-
  • Kallidin