Inhibition of NF-kappa B activation in vitro and in vivo: role of 26S proteasome

Methods Enzymol. 1999;300:345-63. doi: 10.1016/s0076-6879(99)00140-8.

Abstract

It is becoming increasingly apparent that NF-kappa B plays a critical role in regulating the inflammatory response. Data obtained from studies in our laboratories demonstrate that the proteasome plays an important role in the inflammatory cascade by regulating the activation of NF-kappa B. Indeed, the availability of selective and orally active proteasome inhibitors should prove useful in delineating the roles of the proteasome and NF-kappa B in other pathophysiological conditions such as cancer and heart disease.

MeSH terms

  • Acetylcysteine / analogs & derivatives
  • Acetylcysteine / pharmacology
  • Animals
  • Arthritis / drug therapy
  • Boronic Acids / pharmacology
  • Cell Adhesion Molecules / biosynthesis
  • Cytokines / biosynthesis
  • Dipeptides / pharmacology
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / metabolism
  • Female
  • HeLa Cells
  • Humans
  • Hypersensitivity, Delayed / drug therapy
  • Jurkat Cells
  • Leupeptins / pharmacology
  • NF-kappa B / metabolism*
  • Peptide Hydrolases / drug effects*
  • Protease Inhibitors / pharmacology*
  • Proteasome Endopeptidase Complex*
  • Rats
  • Rats, Inbred Lew
  • T-Lymphocytes / drug effects

Substances

  • Boronic Acids
  • Cell Adhesion Molecules
  • Cytokines
  • Dipeptides
  • Leupeptins
  • NF-kappa B
  • PS 273
  • Protease Inhibitors
  • lactacystin
  • Peptide Hydrolases
  • Proteasome Endopeptidase Complex
  • ATP dependent 26S protease
  • benzyloxycarbonylleucyl-leucyl-leucine aldehyde
  • Acetylcysteine