Evidence that Tn5565, which includes the enterotoxin gene in Clostridium perfringens, can have a circular form which may be a transposition intermediate

FEMS Microbiol Lett. 1999 Jan 1;170(1):281-6. doi: 10.1111/j.1574-6968.1999.tb13385.x.

Abstract

The Clostridium perfringens enterotoxin gene is on a transposon-like element, Tn5565, integrated in the chromosome in human food poisoning strains. The flanking IS elements, IS1470 A and B, are related to IS30. The IS element found in the transposon, IS1469, is related to IS200 and has been found upstream of cpe in all Type A strains. PCR and sequencing studies from cell extracts and plasmid isolations of C. perfringens indicate that Tn5565 can form a circular form with the tandem repeat (IS1470)2, similar to the transposition intermediates described for a number of IS elements.

MeSH terms

  • Base Sequence
  • Clostridium perfringens / genetics*
  • DNA Transposable Elements*
  • DNA, Circular / genetics*
  • Enterotoxins / genetics*
  • Genes, Bacterial
  • Molecular Sequence Data
  • Plasmids / genetics
  • Polymerase Chain Reaction / methods
  • Sequence Analysis, DNA

Substances

  • DNA Transposable Elements
  • DNA, Circular
  • Enterotoxins
  • enterotoxin, Clostridium