Elevated serum tumor necrosis factor-alpha concentrations and bioactivity in Type 2 diabetics and patients with android type obesity

Diabetes Res Clin Pract. 1998 Dec;42(3):169-74. doi: 10.1016/s0168-8227(98)00109-0.


The role of tumor necrosis factor-alpha in insulin resistance has been studied in 59 patients with Type 2 diabetes, 28 with android type obesity and 35 healthy lean controls. Immunoreactive concentrations and bioactivity of serum tumor necrosis factor-alpha have repeatedly been determined in 8 weeks intervals for 12 months, five times per patients, by using ELISA and L929 cell cytotoxicity bioassay. Significantly higher immunoreactive tumor necrosis factor-alpha concentrations and bioactivity have been found in both, the Type 2 diabetic and obese groups as compared to the healthy persons. Tumor necrosis factor-alpha concentrations and bioactivity have showed a significant positive linear correlation with the elevated basal serum C-peptide levels and body mass indexes in both groups of patients. According to these data the cytokine might play a role in insulin resistance in obesity as well in Type 2 diabetes.

MeSH terms

  • Adipose Tissue / anatomy & histology
  • Aged
  • Biomarkers / blood
  • Body Mass Index
  • C-Peptide / blood
  • Diabetes Mellitus / blood*
  • Diabetes Mellitus / physiopathology
  • Diabetes Mellitus, Type 2 / blood*
  • Diabetes Mellitus, Type 2 / physiopathology
  • Female
  • Glucagon / blood
  • Humans
  • Male
  • Middle Aged
  • Obesity / blood*
  • Obesity / physiopathology
  • Reference Values
  • Tumor Necrosis Factor-alpha / analysis
  • Tumor Necrosis Factor-alpha / metabolism*


  • Biomarkers
  • C-Peptide
  • Tumor Necrosis Factor-alpha
  • Glucagon