Separation and detection of the alpha- and beta-chains of hemoglobin of a single intact red blood cells using capillary electrophoresis/electrospray ionization time-of-flight mass spectrometry

P Cao; M Moini

doi:10.1016/s1044-0305(98)00146-9

Separation and detection of the alpha- and beta-chains of hemoglobin of a single intact red blood cells using capillary electrophoresis/electrospray ionization time-of-flight mass spectrometry

J Am Soc Mass Spectrom. 1999 Feb;10(2):184-6. doi: 10.1016/s1044-0305(98)00146-9.

Authors

P Cao¹, M Moini

Affiliation

¹ Department of Chemistry and Biochemistry, University of Texas at Austin 78712, USA.

PMID: 9926410
DOI: 10.1016/s1044-0305(98)00146-9

Abstract

A single intact red blood cell (erythrocyte) was injected into a capillary electrophoresis column, and following in-capillary lysing the alpha- and beta-chains of the hemoglobin (approximately 450 amol) were separated and detected using capillary electrophoresis/electrospray ionization time-of-flight mass spectrometry. The mass specta of the electropherogram peaks of the alpha and beta chains showed identifiable peaks corresponding to multiply protonated and sodiated alpha- and beta-chains of hemoglobin.

MeSH terms

Electrophoresis, Capillary
Erythrocytes / chemistry*
Hemoglobins / analysis*
Hemoglobins / isolation & purification
Humans
Mass Spectrometry

Substances

Hemoglobins