Expression of alphaB-crystallin in a mouse model of inherited retinal degeneration

Neuroreport. 1998 Dec 21;9(18):4161-5. doi: 10.1097/00001756-199812210-00030.


AlphaB-crystallin, which is abundantly expressed in the lens but also in a diversity of other tissues, functions as a stress-inducible molecular chaperone and is increased in brain neurodegenerative diseases. We compared retinal alphaB-crystallin expression in a model of inherited retinal degeneration, the rd mouse, and controls. Northern and in situ hybridization analysis showed alphaB-crystallin mRNA to have an altered spatio-temporal pattern with increased levels localized to glial cells in the degenerative state. Immunocytochemistry confirmed increased expression at Müller cells and astrocytes, together with transiently increased localization to the degenerating photoreceptors. These findings suggest that increased alphaB-crystallin expression is associated with glial cell reaction to neuronal damage in the retina, and may comprise part of the retina's overall defensive response to the stress of apoptotic photoreceptor cell death.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Crystallins / genetics
  • Crystallins / metabolism*
  • Immunohistochemistry
  • In Situ Hybridization
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains / genetics
  • RNA, Messenger / metabolism
  • Reference Values
  • Retina / metabolism
  • Retina / pathology
  • Retinal Degeneration / genetics*
  • Retinal Degeneration / metabolism*
  • Retinal Degeneration / pathology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tissue Distribution


  • Crystallins
  • RNA, Messenger