Identification and characterization of RNA editing events within the 5-HT2C receptor

Ann N Y Acad Sci. 1998 Dec 15;861:38-48. doi: 10.1111/j.1749-6632.1998.tb10171.x.

Abstract

RNA editing is a post-transcriptional modification that generates an RNA transcript with a nucleotide sequence different from its gene. We have recently discovered RNA editing events, involving the conversion of adenosine bases to inosine residues, within the RNA encoding the serotonin 2C (5-HT2C) receptor. Editing events at four major positions, termed A, B, C and D, as well as one minor site termed C', are predicted to alter amino acids within the second intracellular loop of the G-protein coupled 5-HT2C receptor. Editing is mediated by at least two members of a family of adenosine deaminases and is contingent upon the presence of an extensive RNA duplex structure formed by exonic and intronic sequences of 5-HT2C receptor precursor messenger RNA (pre-mRNA). This critical secondary structure has been observed within brain pre-mRNA derived from four species; the isolation of edited 5-HT2C receptor transcripts from these samples further confirms the evolutionary conservation of this RNA processing event. Among members of the 5-HT2 receptor family, editing within second intracellular loop RNA is unique to the 5-HT2C receptor. Editing within the 5-HT2C receptor generates receptor isoforms that differ in their ability to interact with the phospholipase C signaling cascade in a transfected cell line, suggesting that this RNA processing event may contribute to the modulation of serotonergic neurotransmission in the central nervous system.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / metabolism
  • Evolution, Molecular
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Point Mutation
  • RNA Editing*
  • RNA Precursors / chemistry
  • RNA Precursors / genetics
  • Rats
  • Receptor, Serotonin, 5-HT2C
  • Receptors, Serotonin / genetics*
  • Transcription, Genetic

Substances

  • RNA Precursors
  • Receptor, Serotonin, 5-HT2C
  • Receptors, Serotonin