Mouse 5-HT2B receptor-mediated serotonin trophic functions

Ann N Y Acad Sci. 1998 Dec 15;861:67-73. doi: 10.1111/j.1749-6632.1998.tb10174.x.

Abstract

5-HT2B receptors, in addition to phospholipase C stimulation, are able to trigger activation of the proto-oncogene product p21ras. During mouse embryogenesis, a peak of 5-HT2B receptor expression is detected at the neurulation stage; we localized the 5-HT2B expression in neural crest cells, heart myocardium, and somites. The requirement for functional 5-HT2B receptors shortly after gastrulation, is supported by culture of embryos exposed to 5-HT2B-high affinity antagonist such as ritanserin, which induces morphological defects in the cephalic region, heart and neural tube. Functional 5-HT2B receptors are also expressed during the serotonergic differentiation of the mouse F9 teratocarcinoma-derived clonal cell line 1C11. Upon 2 days of induction by cAMP, 5-HT2B receptors become functional, and on day 4, the appearance of 5-HT2A receptors coincides with the onset of active serotonin transporter by these cells. Active serotonin uptake is modulated by serotonin suggesting autoreceptor functions for 5-HT2B receptors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cell Differentiation
  • Embryonic and Fetal Development*
  • Gene Expression Regulation, Developmental*
  • Genes, ras
  • Mice
  • Proto-Oncogene Proteins p21(ras) / genetics
  • Receptor, Serotonin, 5-HT2B
  • Receptors, Serotonin / genetics*
  • Receptors, Serotonin / physiology*
  • Serotonin / physiology*

Substances

  • Receptor, Serotonin, 5-HT2B
  • Receptors, Serotonin
  • Serotonin
  • Proto-Oncogene Proteins p21(ras)