Epitope tagging

Annu Rev Genet. 1998;32:601-18. doi: 10.1146/annurev.genet.32.1.601.

Abstract

Epitope tagging is a recombinant DNA method by which a protein encoded by a cloned gene is made immunoreactive to a known antibody. This review discusses the major advantages and limitations of epitope tagging and describes a number of recent applications. Major areas of application include monitoring protein expression, localizing proteins at the cellular and subcellular levels, and protein purification, as well as the analysis of protein topology, dynamics and interactions. Recently the method has also found use in transgenic and gene therapy studies and in the emerging fields of functional genomics and proteomics.

Publication types

  • Review

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibody Specificity
  • DNA, Recombinant / genetics
  • Epitopes / genetics*
  • Genetic Techniques
  • Immunologic Techniques
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology

Substances

  • DNA, Recombinant
  • Epitopes
  • Recombinant Proteins