Expression of a pulmonary endothelial norepinephrine transporter

J Neural Transm (Vienna). 1998;105(10-12):1187-91. doi: 10.1007/s007020050121.


To determine whether pulmonary endothelial cells express the plasma membrane norepinephrine transporter (NET), an ovine-specific riboprobe was prepared from a partial NET cDNA clone isolated by PCR from a placental cDNA library. Northern hybridization detected a predominant 5.8 kb and a weaker transcript at 3.6 kb in RNAs isolated from near-term ovine fetal pulmonary endothelial cells. These data should be useful for future studies examining the molecular mechanisms underlying pulmonary endothelial NET expression and regulation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Carrier Proteins / metabolism*
  • Endothelium, Vascular / metabolism*
  • Gene Library
  • Humans
  • Lung / blood supply*
  • Molecular Sequence Data
  • Norepinephrine / metabolism*
  • Norepinephrine Plasma Membrane Transport Proteins
  • Polymerase Chain Reaction
  • Sequence Homology, Nucleic Acid
  • Sheep
  • Symporters*


  • Carrier Proteins
  • Norepinephrine Plasma Membrane Transport Proteins
  • SLC6A2 protein, human
  • Symporters
  • Norepinephrine