Activation of phospholipase C-Ca2+ system by sphingosine 1-phosphate in CHO cells transfected with Edg-3, a putative lipid receptor

FEBS Lett. 1999 Jan 22;443(1):25-30. doi: 10.1016/s0014-5793(98)01676-7.


Sphingosine 1-phosphate (S1P) induces phospholipase C (PLC) activation and Ca2+ mobilization in many types of cells. We examined the possible involvement of Edg-3, one of the putative S1P receptors, in the phospholipase C (PLC)-Ca2+ system. S1P increased the cytoplasmic free Ca2+ concentration without detectable inositol phosphate production in vector-transfected CHO cells. In the Edg-3-transfected cells, however, the S1P-induced Ca2+ response was clearly enhanced, which was associated with a significant production of inositol phosphate. These S1P-induced responses in the Edg-3-transfected cells were inhibited by U73122, a potent PLC inhibitor. We conclude that Edg-3 may be one of the S1P receptors participating in the activation of the PLC-Ca2+ system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CHO Cells
  • Calcium Signaling*
  • Cricetinae
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Enzyme Activation
  • I-kappa B Proteins*
  • Inositol Phosphates / metabolism
  • Lysophospholipids*
  • NF-KappaB Inhibitor alpha
  • Receptors, Cell Surface / metabolism*
  • Receptors, Lysophospholipid
  • Recombinant Proteins / metabolism
  • Sphingosine / analogs & derivatives*
  • Sphingosine / metabolism
  • Transfection
  • Type C Phospholipases / metabolism*
  • Uridine Triphosphate / metabolism
  • Virulence Factors, Bordetella


  • DNA-Binding Proteins
  • I-kappa B Proteins
  • Inositol Phosphates
  • Lysophospholipids
  • Receptors, Cell Surface
  • Receptors, Lysophospholipid
  • Recombinant Proteins
  • Virulence Factors, Bordetella
  • NF-KappaB Inhibitor alpha
  • sphingosine 1-phosphate
  • Type C Phospholipases
  • Sphingosine
  • Uridine Triphosphate