Isolation and expression analysis of a novel human homologue of the Drosophila glial cells missing (gcm) gene

FEBS Lett. 1999 Jan 15;442(2-3):151-6. doi: 10.1016/s0014-5793(98)01650-0.


A novel human homologue (GCMB) of the Drosophila glial cells missing gene (dGCM) was isolated using RACE. GCMB contained a gcm motif sequence and a nuclear targeting sequence similar to that of dGCM and mouse GCMb. Homology searches indicated that GCMB was located within chromosome 6p24.2. Transcripts of GCMB were detected by means of RT-PCR in fetal brain, normal adult kidney, 3/3 medulloblastomas, 1/3 gliomas and 4/8 non-neuroepithelial tumor cell lines. Our data suggest that humans have two homologues of gcm like mice and that human gcm genes form a novel family which may function not only during fetal development but also in the postnatal or pathological stage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Brain / embryology
  • Brain / metabolism*
  • Chromosomes, Human, Pair 6 / genetics
  • Cloning, Molecular*
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Drosophila melanogaster / genetics
  • Expressed Sequence Tags
  • Gene Expression*
  • Gene Library
  • Humans
  • Kidney / metabolism
  • Molecular Sequence Data
  • Neoplasms, Neuroepithelial / metabolism
  • Neuropeptides / chemistry
  • Neuropeptides / genetics*
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Trans-Activators / chemistry
  • Trans-Activators / genetics*
  • Transcription Factors
  • Tumor Cells, Cultured


  • DNA-Binding Proteins
  • Drosophila Proteins
  • GCM protein, Drosophila
  • Neuropeptides
  • Trans-Activators
  • Transcription Factors

Associated data

  • GENBANK/AF079550