Molecular cloning, mapping, and characterization of a novel human gene, MTA1-L1, showing homology to a metastasis-associated gene, MTA1

J Hum Genet. 1999;44(1):52-6. doi: 10.1007/s100380050107.

Abstract

Through large-scale sequencing of clones randomly selected from libraries of human cDNAs, we have isolated a novel human gene encoding a product with 59.6% identity in amino acid sequence to human MTA1, a protein associated with tumor invasion and metastasis. This cDNA, named MTA1-L1 (MTA1 like 1), consists of 2736 nucleotides with an open reading frame encoding 668 amino acids. A single 3.0-kb transcript of MTA1-L1 was expressed ubiquitously on Northern blots. Structural analysis of the MTA1-L1 gene revealed 18 exons spanning 8.1 kb of genomic DNA. We assigned the MTA1-L1 locus to chromosomal band 11q12-13.1 by fluorescence in situ hybridization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11 / genetics
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Histone Deacetylases*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • Neoplasm Metastasis / genetics*
  • Proteins / genetics*
  • Repressor Proteins*
  • Sequence Homology, Amino Acid
  • Trans-Activators

Substances

  • DNA, Complementary
  • Mta1 protein, human
  • Proteins
  • Repressor Proteins
  • Trans-Activators
  • Histone Deacetylases

Associated data

  • GENBANK/AB016591