Coupled-column liquid chromatographic analysis of catecholamines, serotonin, and metabolites in human urine

Clin Chem. 1999 Feb;45(2):262-8.


A column-switching HPLC system was utilized for the simultaneous determination of epinephrine, norepinephrine, dopamine, serotonin, and their metabolites metanephrine, normetanephrine, 3, 4-dihydroxyphenylacetic acid, homovanillic acid, and 5-hydroxyindoleacetic acid in human urine. The sample was injected directly onto a C18-alkyl-diol silica precolumn, which separated the analytes from matrix. The analytes were eluted from the precolumn onto the analytical column by the use of column-switching techniques and were then separated on the analytical column by means of ion-pair reversed-phase HPLC. The analytes were then oxidized to the corresponding quinones and converted into fluorescent derivatives by reaction with meso-1,2-diphenylethylenediamine.

MeSH terms

  • Case-Control Studies
  • Catecholamines / urine*
  • Chromatography, High Pressure Liquid / instrumentation*
  • Humans
  • Pheochromocytoma / urine
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Serotonin / urine*
  • Spectrometry, Fluorescence


  • Catecholamines
  • Serotonin