Systemic immune response after intravesical instillation of bacille Calmette-Guérin (BCG) for superficial bladder cancer

Clin Exp Immunol. 1999 Jan;115(1):131-5. doi: 10.1046/j.1365-2249.1999.00756.x.


The mechanism of anti-tumour activity by BCG is not known clearly. However, many studies suggest that immunological response is related to effectiveness of intravesical instillation of BCG in the therapy for superficial bladder carcinoma. Peripheral blood mononuclear cells (PBMC), urine and serum were obtained from patients with superficial carcinoma at various times during the course of BCG instillation. Urine of patients showed increased levels of IL-1beta, IL-2, IL-6, tumour necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma) and macrophage colony-stimulating factor (M-CSF) after BCG instillation. Levels of IL-2 and IFN-gamma in the serum also increased after BCG instillation, but IL-1beta, IL-6, TNF-alpha and M-CSF were not detectable. Maximal levels of IL-2 and IFN-gamma in the urine or serum were shown after the fourth instillation. BCG-induced killer cell activity in PBMC increased significantly after the third BCG instillation. These results suggest that BCG instillation involved not only local immunological efforts but also systemic immune responses. Tumour-free patients produced higher BCG-induced killer cell activity than tumour recurrence patients. BCG-induced killer cell activity may be useful for monitoring the effectiveness of intravesical BCG instillation.

MeSH terms

  • Administration, Intravesical
  • Adult
  • Aged
  • Aged, 80 and over
  • BCG Vaccine / administration & dosage*
  • Cytokines / blood
  • Cytokines / urine
  • Female
  • Humans
  • Interferon-gamma / blood
  • Interferon-gamma / urine
  • Interleukin-1 / blood
  • Interleukin-1 / urine
  • Interleukin-2 / blood
  • Interleukin-2 / urine
  • Interleukin-6 / blood
  • Interleukin-6 / urine
  • Killer Cells, Natural / immunology
  • Leukocytes, Mononuclear / immunology
  • Macrophage Colony-Stimulating Factor / blood
  • Macrophage Colony-Stimulating Factor / urine
  • Male
  • Middle Aged
  • Tumor Necrosis Factor-alpha / analysis
  • Tumor Necrosis Factor-alpha / urine
  • Urinary Bladder Neoplasms / blood
  • Urinary Bladder Neoplasms / therapy*


  • BCG Vaccine
  • Cytokines
  • Interleukin-1
  • Interleukin-2
  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • Macrophage Colony-Stimulating Factor
  • Interferon-gamma