Distinct clonal origin of low-grade MALT-type and high-grade lesions of a multifocal gastric lymphoma

Histopathology. 1999 Jan;34(1):6-8. doi: 10.1046/j.1365-2559.1999.00568.x.


Aims: Low-grade mucosa-associated lymphoid tissue (MALT) lymphoma and high-grade B-cell non-Hodgkin's lymphoma (NHL) of the stomach may occur simultaneously. To determine the clonal relationship between these tumours, we compared the immunoglobulin heavy chain gene (IgH) rearrangements of low and high-grade components of a multifocal gastric NHL.

Methods and results: The complementary determining region 3 (CDR3) of the IgH gene rearrangements were polymerase chain reaction (PCR) amplified, cloned and sequenced. The analysis of the CDR3 sequences rearranged by tumour cells of low-grade MALT and the high-grade NHL revealed different nucleic acid sequences.

Conclusion: These findings suggest that low-grade MALT and high-grade B-cell components of multifocal gastric NHL may represent unrelated clones.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Clone Cells
  • Female
  • Gene Rearrangement
  • Humans
  • Immunoglobulin Heavy Chains / genetics
  • Lymphoma, B-Cell / immunology*
  • Lymphoma, B-Cell, Marginal Zone / immunology*
  • Lymphoma, Non-Hodgkin / immunology*
  • Middle Aged
  • Molecular Sequence Data
  • Neoplasms, Multiple Primary / genetics
  • Sequence Homology, Nucleic Acid
  • Stomach Neoplasms / immunology*


  • Immunoglobulin Heavy Chains