Viability of isolated single hair follicles preserved at 4 degrees C

Dermatol Surg. 1999 Jan;25(1):26-9. doi: 10.1046/j.1524-4725.1999.07319.x.


Background: Hair follicle preservation for the purpose of delayed application would help us to transplant hair follicles more efficiently.

Methods: Isolated single hair follicles were preserved at 4 degrees C in four different solutions. Viability of preserved follicles was judged by organ culture and cell culture. In addition, a small number of hair follicles were transplanted into athymic mice. RESULTS. By cell culture, both dermal papilla and outer root sheath cells could be cultivated after 7 days of preservation. Hair follicles preserved for 48 hours showed a significant increase of hair shafts in organ culture. Those preserved for 7 days regrew well when transplanted into athymic mice.

Conclusion: Preservation of hair follicles at 4 degrees C could be one option to prepare many follicular units at one time for transplantation.

MeSH terms

  • Adult
  • Animals
  • Cell Culture Techniques
  • Cell Survival
  • Female
  • Hair Follicle / anatomy & histology
  • Hair Follicle / physiology*
  • Hair Follicle / transplantation*
  • Humans
  • Immunohistochemistry
  • Male
  • Mice
  • Mice, Nude
  • Organ Culture Techniques
  • Organ Preservation*
  • Temperature