Glutathione S-transferase in human endometrium: quantitation and interindividual variability in isoform content

Gynecol Obstet Invest. 1999;47(2):114-9. doi: 10.1159/000010073.

Abstract

Objectives: The goals of the present study were to determine whether human endometrium contains glutathione S-transferases (GSTs), to identify the isoforms present (if any) and to assess the causes of any intersubject variability which was observed.

Method: Hysterectomy patients (n = 32, 13 with endometrial carcinoma) were examined in the core study. The ability of cytosol from these endometria to conjugate 1-chloro-2,4-dinitrobenzene was measured. Additional studies with chemical inhibitors and monoclonal antibodies to GSTpi were also performed.

Results: The pattern of inhibition caused by ethacrynic acid and cibacron blue strongly suggests substantial interindividual variability in type (alpha and pi dominant) and amount ( approximately 10-fold) of this important group of enzymes. Significantly lower GST activity was seen in cancer patients who were smokers when compared with all other subjects (p < 0.05).

Conclusion: There are substantial interindividual differences in the GST content of human endometrium. Furthermore, lower GST activity in tissues from endometrial carcinoma patients with a history of smoking may have contributed to the neoplastic transformation of this tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cytosol / enzymology
  • Endometrium / enzymology*
  • Enzyme Inhibitors / pharmacology
  • Ethacrynic Acid / pharmacology
  • Female
  • Glutathione Transferase / analysis*
  • Glutathione Transferase / antagonists & inhibitors
  • Humans
  • Hysterectomy
  • Isoenzymes / analysis*
  • Isoenzymes / antagonists & inhibitors
  • Smoking / adverse effects
  • Triazines / pharmacology
  • Uterine Cervical Neoplasms / enzymology
  • Uterine Neoplasms / enzymology

Substances

  • Enzyme Inhibitors
  • Isoenzymes
  • Triazines
  • Cibacron Blue F 3GA
  • Glutathione Transferase
  • Ethacrynic Acid