Gene expression of the GATA-3 transcription factor is increased in atopic asthma

J Allergy Clin Immunol. 1999 Feb;103(2 Pt 1):215-22. doi: 10.1016/s0091-6749(99)70493-8.


Background: High expression of IL-5 by T cells in the airways of asthmatic individuals is believed to play a fundamental role in the eosinophilia associated with this disease. Recently, the transcription factor GATA-3 was shown to be critical for IL-5 gene expression in TH2 cells in vitro.

Objective: Our aim was to examine the expression of GATA-3 mRNA and its colocalization within the airways of asthmatic and nonasthmatic individuals.

Methods: We investigated the association between GATA-3 gene expression, airway inflammatory cells, and IL-5 gene expression in bronchoalveolar lavage fluid and bronchial biopsy specimens from atopic asthmatic subjects (n = 10) and normal control subjects (n = 10).

Results: We report that GATA-3 mRNA expression is significantly increased in the airways of asthmatic subjects compared with those of normal control subjects (P <.001). Numbers of cells expressing GATA-3 transcripts correlated significantly with reduced airway caliber (P <.05) and airways hyperresponsiveness (P <.05) in asthmatic subjects. Colocalization studies showed that the majority (approximately 60% to 90%) of GATA-3 mRNA+ cells in asthmatic airways were CD3(+) T cells, with smaller contributions from major basic protein+ eosinophils and tryptase+ mast cells. The density of GATA-3 mRNA+ cells correlated significantly with the numbers of cells expressing IL-5 mRNA (P <.001, r = 0.879 for bronchoalveolar lavage fluid; P <. 05, r = 0.721 for biopsy specimens). Furthermore, double in situ hybridization demonstrated that approximately 76% of GATA-3 mRNA+ cells coexpressed IL-5 mRNA and that 91% of IL-5 mRNA+ cells coexpressed GATA-3 mRNA.

Conclusion: The results of this study provide the first evidence of increased GATA-3 gene expression in association with IL-5 mRNA+ cells in asthmatic airways. These findings support a causal association between augmented GATA-3 expression and dysregulated IL-5 expression in atopic asthma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Asthma / metabolism*
  • Bronchi / metabolism
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / genetics*
  • Female
  • GATA3 Transcription Factor
  • Gene Expression Regulation / immunology*
  • Humans
  • Hypersensitivity, Immediate / metabolism*
  • Immunochemistry
  • In Situ Hybridization
  • Interleukin-5 / biosynthesis
  • Male
  • Middle Aged
  • Phenotype
  • RNA, Messenger / metabolism
  • Trachea / metabolism
  • Trans-Activators / biosynthesis
  • Trans-Activators / genetics*
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics*


  • DNA-Binding Proteins
  • GATA3 Transcription Factor
  • GATA3 protein, human
  • Interleukin-5
  • RNA, Messenger
  • Trans-Activators
  • Transcription Factors