Estrogen enhances immunoglobulin production by human PBMCs

J Allergy Clin Immunol. 1999 Feb;103(2 Pt 1):282-8. doi: 10.1016/s0091-6749(99)70503-8.

Abstract

Background: It has been suggested that estrogen may enhance humoral immune responses and may be involved in the pathogenesis of autoimmune diseases.

Objective: We studied the in vitro effects of 17beta-estradiol (E2 ) on spontaneous immunoglobulin production by human PBMCs.

Methods: PBMCs from healthy human volunteers were cultured with E2. Levels of IgG and IgM and cytokine activity were measured by ELISA. Proliferation was determined by [3H]-thymidine uptake. The cell viability was assessed by a trypan blue exclusion test.

Results: E2 enhanced IgG and IgM production of PBMCs both from men and women without altering cell viability and proliferation. The stimulatory effect of E2 was revealed at 10(-10) mol/L, increased in a dose-dependent fashion, and was maximized at 10(-8) mol/L. IgG production of PBMCs in the presence of E2 (10(-8) mol/L) was 220% of control cells, and that of IgM was 211%. Immunoglobulin production of E2 -treated B cells was slightly higher than that of control cells; IgG production was 161% of control cells, and that of IgM was 157%. Anti-IL-10 antibody partially blocked the E2 effect on immunoglobulin production of PBMCs; anti-IL-10 reduced IgG production in the presence of E2 from 206% to 154% of control cells, and that of IgM from 206% to 152%. E2 increased IL-10 production of monocytes up to 250% of control level, but it did not affect that of T cells or B cells. Exogenous IL-10 (1 U/mL) further enhanced E2 -induced increase of immunoglobulin production by B cells, although this level of IL-10 alone was ineffective for B cells.

Conclusion: These results suggest that E2 may increase immunoglobulin production of human PBMCs mainly by increasing IL-10 production of monocytes. The results also support that E2 may act as an important stimulator for human humoral immunity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / pharmacology*
  • Adult
  • B-Lymphocytes / drug effects
  • B-Lymphocytes / metabolism
  • Cytokines / biosynthesis
  • Cytokines / immunology
  • Estradiol / pharmacology*
  • Female
  • Humans
  • Immune Sera / pharmacology
  • Immunoglobulin G / biosynthesis*
  • Immunoglobulin M / biosynthesis*
  • Interleukin-10 / pharmacology
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / metabolism*
  • Male
  • Middle Aged

Substances

  • Adjuvants, Immunologic
  • Cytokines
  • Immune Sera
  • Immunoglobulin G
  • Immunoglobulin M
  • Interleukin-10
  • Estradiol