The antibacterial action of the oil extract obtained from tomato pulp has been studied with the use of museum strains Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 5638-P and Candida albicans ATCC 885-653, recommended for the determination of the antibacterial activity of medicinal preparations (State Pharmacopoeia, edition XI), as well as Enterobacter, Streptococcus, Staphylococcus, Klebsiella and Escherichia clinical strains. As revealed in this study, tomato pulp oil extract produces a wide-spectrum antibacterial effect on Gram-positive and Gram-negative microorganisms and on fungi of the genus Candida. The study has determined that this antibacterial action is linked with the presence of a complex of organic acids (succinic, citric, tartaric, etc.).