Inhibitory effect of Trapidil on the proliferation of bovine corneal fibroblasts in vitro

Graefes Arch Clin Exp Ophthalmol. 1999 Jan;237(1):72-7. doi: 10.1007/s004170050197.

Abstract

Background: In order to develop new strategies for the pharmacological modulation of posttraumatic and postsurgical wound healing of the corneal stroma, the effect of Trapidil, a competitive platelet-derived growth factor (PDGF) antagonist, on the proliferation of cultured bovine stromal fibroblasts (BSF) was investigated.

Methods: BSF, obtained from explant cultures, were seeded at a cell density of 100/mm2. The effect of various concentrations of Trapidil on cell viability and cell proliferation was determined using three different culture conditions: (1) serum-free medium (WM/F12), (2) serum-containing medium (WM/F12 + 10% FCS), and (3) serum-free medium + 50 ng/ml PDGF-BB. Trapidil was added in concentrations ranging from 100 micrograms/ml to 400 micrograms/ml. Cell numbers were determined 2 and 5 days after addition of Trapidil, using a computer-based cell-counting system. Cell viability was evaluated morphologically and by means of a repopulation assay.

Results: Addition of Trapidil (100-400 micrograms/ml) led to a significant, dose-dependent inhibition of both serum- and PDGF-BB-induced proliferation of BSF. In contrast, treatment of quiescent BSF, cultured in serum-free medium, did not result in a significant reduction of cell number. No cytotoxic effects were observed.

Conclusion: The results of the present study demonstrate an inhibitory effect of Trapidil on the proliferation of BSF. It can be assumed that application of Trapidil might be a useful tool in the prevention of corneal complications after trauma (e.g., scarring, astigmatism and--with respect to photorefractive procedures--formation of haze and regression of the refractive effect).

MeSH terms

  • Animals
  • Cattle
  • Cell Count
  • Cell Division / drug effects
  • Cell Survival / drug effects
  • Cells, Cultured
  • Corneal Stroma / cytology
  • Corneal Stroma / drug effects*
  • Dose-Response Relationship, Drug
  • Fibroblasts / cytology*
  • Platelet Aggregation Inhibitors / pharmacology*
  • Trapidil / pharmacology*

Substances

  • Platelet Aggregation Inhibitors
  • Trapidil