Abstract
Nicotianamine synthase (NAS), the key enzyme in the biosynthetic pathway for the mugineic acid family of phytosiderophores, catalyzes the trimerization of S-adenosylmethionine to form one molecule of nicotianamine. We purified NAS protein and isolated the genes nas1, nas2, nas3, nas4, nas5-1, nas5-2, and nas6, which encode NAS and NAS-like proteins from Fe-deficient barley (Hordeum vulgare L. cv Ehimehadaka no. 1) roots. Escherichia coli expressing nas1 showed NAS activity, confirming that this gene encodes a functional NAS. Expression of nas genes as determined by northern-blot analysis was induced by Fe deficiency and was root specific. The NAS genes form a multigene family in the barley and rice genomes.
MeSH terms
-
Alkyl and Aryl Transferases / genetics*
-
Alkyl and Aryl Transferases / isolation & purification
-
Alkyl and Aryl Transferases / metabolism
-
Amino Acid Sequence
-
Azetidinecarboxylic Acid / analogs & derivatives
-
Azetidinecarboxylic Acid / metabolism
-
Base Sequence
-
Cloning, Molecular
-
DNA Primers / genetics
-
DNA, Complementary / genetics
-
DNA, Plant / genetics
-
Escherichia coli / genetics
-
Gene Expression
-
Genes, Plant*
-
Hordeum / enzymology
-
Hordeum / genetics
-
Iron / metabolism
-
Molecular Sequence Data
-
Multigene Family
-
Oryza / enzymology
-
Oryza / genetics
-
Sequence Homology, Amino Acid
-
Siderophores / biosynthesis
Substances
-
DNA Primers
-
DNA, Complementary
-
DNA, Plant
-
Siderophores
-
nicotianamine
-
Azetidinecarboxylic Acid
-
Iron
-
Alkyl and Aryl Transferases
-
nicotianamine synthase
Associated data
-
GENBANK/AB010086
-
GENBANK/AB011264
-
GENBANK/AB011265
-
GENBANK/AB011266
-
GENBANK/AB011267
-
GENBANK/AB011268
-
GENBANK/AB011269