Cloning and nucleotide sequence of the gene encoding a serine proteinase inhibitor named marinostatin from a marine bacterium, Alteromonas sp. strain B-10-31

Biosci Biotechnol Biochem. 1998 Dec;62(12):2446-9. doi: 10.1271/bbb.62.2446.

Abstract

The gene (mstI) encoding a serine proteinase inhibitor named marinostatin from marine Alteromonas sp. strain B-10-31 was cloned and its nucleotide sequence was analyzed. A short open reading frame of 192 bp encoded 63 amino acids with a molecular weight of 6,985. Furthermore, the initial product of marinostatin (marinostatin L) was purified and its amino acid sequence was analyzed. These results indicate that marinostatin is produced as a unique precursor consisting of the mature peptide and the leader peptide for an ATP-binding cassette (ABC) transporter, and furthermore the initial product of marinostatin is dehydrated and processed by proteolysis to give homologous forms of marinostatin.

MeSH terms

  • ATP-Binding Cassette Transporters / chemistry
  • ATP-Binding Cassette Transporters / genetics*
  • Amino Acid Sequence
  • Bacterial Proteins*
  • Base Sequence
  • Cloning, Molecular
  • Consensus Sequence
  • DNA, Bacterial / chemistry
  • Gram-Negative Aerobic Rods and Cocci / enzymology*
  • Gram-Negative Aerobic Rods and Cocci / genetics
  • Molecular Sequence Data
  • Molecular Weight
  • Nucleic Acid Hybridization
  • Open Reading Frames
  • Protein Precursors / chemistry
  • Protein Precursors / genetics*
  • Protein Sorting Signals / chemistry
  • Protein Sorting Signals / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Serine Proteinase Inhibitors / chemistry
  • Serine Proteinase Inhibitors / genetics*

Substances

  • ATP-Binding Cassette Transporters
  • Bacterial Proteins
  • DNA, Bacterial
  • Protein Precursors
  • Protein Sorting Signals
  • Serine Proteinase Inhibitors
  • marinostatin L protein, Alteromonas