Abstract
The gene (mstI) encoding a serine proteinase inhibitor named marinostatin from marine Alteromonas sp. strain B-10-31 was cloned and its nucleotide sequence was analyzed. A short open reading frame of 192 bp encoded 63 amino acids with a molecular weight of 6,985. Furthermore, the initial product of marinostatin (marinostatin L) was purified and its amino acid sequence was analyzed. These results indicate that marinostatin is produced as a unique precursor consisting of the mature peptide and the leader peptide for an ATP-binding cassette (ABC) transporter, and furthermore the initial product of marinostatin is dehydrated and processed by proteolysis to give homologous forms of marinostatin.
MeSH terms
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ATP-Binding Cassette Transporters / chemistry
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ATP-Binding Cassette Transporters / genetics*
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Amino Acid Sequence
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Bacterial Proteins*
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Base Sequence
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Cloning, Molecular
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Consensus Sequence
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DNA, Bacterial / chemistry
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Gram-Negative Aerobic Rods and Cocci / enzymology*
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Gram-Negative Aerobic Rods and Cocci / genetics
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Molecular Sequence Data
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Molecular Weight
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Nucleic Acid Hybridization
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Open Reading Frames
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Protein Precursors / chemistry
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Protein Precursors / genetics*
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Protein Sorting Signals / chemistry
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Protein Sorting Signals / genetics
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Serine Proteinase Inhibitors / chemistry
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Serine Proteinase Inhibitors / genetics*
Substances
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ATP-Binding Cassette Transporters
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Bacterial Proteins
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DNA, Bacterial
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Protein Precursors
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Protein Sorting Signals
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Serine Proteinase Inhibitors
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marinostatin L protein, Alteromonas