Apoptosis in early ocular morphogenesis in the mouse

Brain Res Dev Brain Res. 1999 Jan 11;112(1):129-33. doi: 10.1016/s0165-3806(98)00153-9.


Development of the eye requires complex interactions between tissues, extracellular matrix and growth factors. Most cells of the optic primordia grow and differentiate into discrete ocular structures; however, other cells have death as their developmental fate. The most common mechanisms of cell death are apoptosis and necrosis. We have identified the cell death that occurs during ocular morphogenesis in ZRDCT-N mice as apoptosis. Mouse embryos, ages E8.5-E11.5, were embedded in paraffin, sectioned at 5 microns and stained with hematoxylin or by the terminal deoxytransferase-mediated dUTP-biotin nick end-labeling (TUNEL) method. The spatial and temporal distribution of apoptotic cells was mapped at 0.5 day intervals using a computerized image analysis system, and 3-D reconstructions were made at each embryonic age. Our data indicate that apoptosis plays a role in normal ocular morphogenesis and provides the groundwork for studies of abnormal ocular development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / physiology*
  • Embryo, Mammalian / cytology
  • Embryo, Mammalian / physiology
  • Embryonic and Fetal Development / physiology
  • Eye / cytology
  • Eye / embryology*
  • Image Processing, Computer-Assisted
  • In Situ Nick-End Labeling
  • Mice / embryology
  • Mice, Inbred C57BL