A copper cofactor for the ethylene receptor ETR1 from Arabidopsis

Science. 1999 Feb 12;283(5404):996-8. doi: 10.1126/science.283.5404.996.

Abstract

The ETR1 receptor from Arabidopsis binds the gaseous hormone ethylene. A copper ion associated with the ethylene-binding domain is required for high-affinity ethylene-binding activity. A missense mutation in the domain that renders the plant insensitive to ethylene eliminates both ethylene binding and the interaction of copper with the receptor. A sequence from the genome of the cyanobacterium Synechocystis sp. strain 6803 that shows homology to the ethylene-binding domain of ETR1 encodes a functional ethylene-binding protein. On the basis of sequence conservation between the Arabidopsis and the cyanobacterial ethylene-binding domains and on in vitro mutagenesis of ETR1, a structural model for this copper-based ethylene sensor domain is presented.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Amino Acid Substitution
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Binding Sites
  • Conserved Sequence
  • Copper / analysis
  • Copper / metabolism*
  • Copper Sulfate / pharmacology
  • Cyanobacteria / genetics
  • Cyanobacteria / metabolism
  • Dimerization
  • Ethylenes / metabolism*
  • Models, Molecular
  • Mutagenesis
  • Open Reading Frames
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / isolation & purification
  • Plant Proteins / metabolism*
  • Receptors, Cell Surface / chemistry
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / isolation & purification
  • Receptors, Cell Surface / metabolism*
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae
  • Silver / metabolism
  • Silver / pharmacology

Substances

  • Bacterial Proteins
  • Ethylenes
  • Plant Proteins
  • Receptors, Cell Surface
  • Recombinant Fusion Proteins
  • ethylene receptors, plant
  • Silver
  • Copper
  • ethylene
  • Copper Sulfate