Identification of contaminating fungal DNA sequences in Zymolyase

D Rimek; A P Garg; W H Haas; R Kappe

doi:10.1128/JCM.37.3.830-831.1999

Identification of contaminating fungal DNA sequences in Zymolyase

J Clin Microbiol. 1999 Mar;37(3):830-1. doi: 10.1128/JCM.37.3.830-831.1999.

Authors

D Rimek¹, A P Garg, W H Haas, R Kappe

Affiliation

¹ Hygiene Institute, Children's Hospital, University of Heidelberg, Heidelberg, Germany. dagmar_rimek@ukl.uniheidelberg.de

Abstract

When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu stricto complex and Kluyveromyces lodderae. Lyticase, lysing enzymes, and proteinase K appeared to be free from fungal DNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Primers
DNA, Fungal / genetics
DNA, Fungal / isolation & purification*
DNA, Ribosomal / genetics
DNA, Ribosomal / isolation & purification
Drug Contamination*
Glucan Endo-1,3-beta-D-Glucosidase*
Kluyveromyces / genetics
Kluyveromyces / isolation & purification*
Polymerase Chain Reaction / methods
RNA, Ribosomal, 16S / genetics
Saccharomyces / genetics
Saccharomyces / isolation & purification*

Substances

DNA Primers
DNA, Fungal
DNA, Ribosomal
RNA, Ribosomal, 16S
Glucan Endo-1,3-beta-D-Glucosidase