Msn1p/Mss10p, Mss11p and Muc1p/Flo11p are part of a signal transduction pathway downstream of Mep2p regulating invasive growth and pseudohyphal differentiation in Saccharomyces cerevisiae

Mol Microbiol. 1999 Jan;31(1):103-16. doi: 10.1046/j.1365-2958.1999.01151.x.

Abstract

In Saccharomyces cerevisiae, a network of signal transduction pathways governs the switch from yeast-type growth to pseudohyphal and invasive growth that occurs in response to nutrient limitation. Important elements of this network have been identified, including nutrient signal receptors, GTP-binding proteins, components of the pheromone-dependent MAP kinase cascade and several transcription factors. However, the structural and functional mapping of these pathways is far from complete. Here, we present data regarding three genes, MSN1/MSS10, MSS11 and MUC1/FLO11, which form an essential part of the signal transduction network establishing invasive growth. Both MSN1 and MSS11 are involved in the co-regulation of starch degradation and invasive growth. Msn1p and Mss11p act downstream of Mep2p and Ras2p and regulate the transcription of both STA2 and MUC1. We show that MUC1 mediates the effect of Msn1p and Mss11p on invasive growth. In addition, our results suggest that the activity of Msn1p is independent of the invasive growth MAP kinase cascade, but the Mss11p is required for the activation of pseudohyphal and invasive growth by Ste12p. We also show that starch metabolism in S. cerevisiae is subject to regulation by components of the MAP kinase cascade.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism
  • Carrier Proteins / genetics*
  • Cation Transport Proteins*
  • DNA-Binding Proteins / genetics*
  • Epistasis, Genetic
  • Fungal Proteins / genetics*
  • Gene Expression Regulation, Fungal*
  • Immediate-Early Proteins*
  • Intracellular Signaling Peptides and Proteins
  • MAP Kinase Kinase Kinases / genetics
  • Membrane Glycoproteins
  • Membrane Proteins / genetics*
  • Protein-Serine-Threonine Kinases / genetics
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development*
  • Saccharomyces cerevisiae Proteins*
  • Schizosaccharomyces pombe Proteins*
  • Signal Transduction*
  • Starch / metabolism
  • Transcription Factors*
  • Transcription, Genetic
  • ras Proteins / genetics

Substances

  • Carrier Proteins
  • Cation Transport Proteins
  • DNA-Binding Proteins
  • FLO11 protein, S cerevisiae
  • Fungal Proteins
  • Immediate-Early Proteins
  • Intracellular Signaling Peptides and Proteins
  • MEP2 protein, S cerevisiae
  • MSN1 protein, S cerevisiae
  • MSS11 protein, S cerevisiae
  • Membrane Glycoproteins
  • Membrane Proteins
  • STE12 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • Transcription Factors
  • ste11 protein, S pombe
  • Starch
  • Protein-Serine-Threonine Kinases
  • Calcium-Calmodulin-Dependent Protein Kinases
  • MAP Kinase Kinase Kinases
  • STE20 protein, S cerevisiae
  • Ste11 protein, S cerevisiae
  • ras Proteins