Anaphylaxis increases 8-iso-prostaglandin F2alpha release from guinea-pig lung in vitro

Eur J Pharmacol. 1999 Jan 15;365(1):59-64. doi: 10.1016/s0014-2999(98)00859-0.


8-Iso-prostaglandin F2alpha, release from isolated and perfused guinea-pig lung was measured by radioimmunoassay. 8-Iso-prostaglandin F2alpha release was detectable under basal conditions and increased 10-fold during antigen-induced bronchoconstriction, concomitant with the increase of thromboxane B2 and prostaglandin E2. The anti-8-iso-prostaglandin F2alpha serum used in the radioimmunoassay seems to be quite specific for this compound. Pretreatment of lungs with indomethacin (a non-selective inhibitor of cyclooxygenase) reduced 8-iso-prostaglandin F2alpha release under basal conditions and completely abolished the increase observed during lung anaphylaxis. Pretreatment of lungs with NS 398 (N-[2-cyclohexyl]-4-nitrophenyl methanesulphonamide), a selective inhibitor of cyclooxygenase-2, did not change basal or antigen-induced 8-iso-prostaglandin F2alpha release at all. We conclude that under basal conditions guinea-pig lung perfusates contain low levels of 8-iso-prostaglandin F2alpha-like immunoreactivity, which increase 10-fold during antigen-induced bronchoconstriction. This isoprostane seems to be derived from the cyclooxygenation of arachidonic acid via the constitutive form of cyclooxygenase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphylaxis / physiopathology*
  • Animals
  • Bronchoconstriction / drug effects
  • Cyclooxygenase Inhibitors / pharmacology
  • Dinoprost / analogs & derivatives*
  • Dinoprost / analysis
  • Dinoprost / metabolism
  • Dinoprostone / metabolism
  • F2-Isoprostanes
  • Guinea Pigs
  • In Vitro Techniques
  • Indomethacin / pharmacology
  • Lung / drug effects
  • Lung / metabolism*
  • Male
  • Nitrobenzenes / pharmacology
  • Ovalbumin / pharmacology
  • Sulfonamides / pharmacology
  • Thromboxane B2 / metabolism


  • Cyclooxygenase Inhibitors
  • F2-Isoprostanes
  • Nitrobenzenes
  • Sulfonamides
  • N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide
  • 8-epi-prostaglandin F2alpha
  • Thromboxane B2
  • Ovalbumin
  • Dinoprost
  • Dinoprostone
  • Indomethacin