Three-dimensional partial zona dissection for preimplantation genetic diagnosis and assisted hatching

Fertil Steril. 1999 Feb;71(2):308-13. doi: 10.1016/s0015-0282(98)00452-x.


Objective: To develop a new approach to partial zona dissection of oocytes and embryos for facilitating preimplantation genetic sampling and assisted hatching.

Design: Controlled clinical study.

Setting: Preimplantation genetic diagnosis and IVF program, Reproductive Genetics Institute/IVF Illinois, Chicago, Illinois.

Patient(s): Three hundred forty patients undergoing IVF in whom preimplantation genetic diagnosis or assisted hatching was required.

Intervention(s): Three-dimensional partial zona dissection and conventional partial zona dissection were performed, with the use of a simple microneedle, on embryos before preimplantation genetic sampling or on day 3 of embryo development before ET.

Main outcome measure(s): Pregnancy rate, implantation rate, and ease of preimplantation genetic sampling.

Result(s): A pregnancy rate of 42% and an embryo implantation rate of 17.6% were obtained in the group in which three-dimensional partial zona dissection was performed, compared with a pregnancy rate of 33% and an implantation rate of 14.7% in the control group, which underwent conventional partial zona dissection. Preimplantation genetic sampling can be performed without distortion of the blastomere configuration in the embryo.

Conclusion(s): Three-dimensional partial zona dissection is a safe and simple mechanical means of creating an opening sufficient for the atraumatic removal of material from oocytes and embryos for preimplantation genetic diagnosis and assisted hatching.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial

MeSH terms

  • Dissection
  • Embryo Implantation
  • Female
  • Fertilization in Vitro*
  • Genetic Testing / methods*
  • Humans
  • Pregnancy
  • Pregnancy Rate
  • Preimplantation Diagnosis / methods*
  • Zona Pellucida*