Evidence for protein splicing in the endoplasmic reticulum-Golgi intermediate compartment

Biochim Biophys Acta. 1999 Jan 11;1448(3):507-11. doi: 10.1016/s0167-4889(98)00158-x.

Abstract

We present evidence that the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) is a site for protein splicing of cathepsin C: (i) maturation of the enzyme in COS 7 cells is a two-step process starting within the ERGIC, and (ii) the intermediately processed polypeptide retains both termini of the proenzyme and lacks an internal fragment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites / genetics
  • COS Cells
  • Cathepsin C
  • Cell Compartmentation
  • Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / genetics
  • Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / metabolism
  • Endoplasmic Reticulum / metabolism*
  • Enzyme Precursors / genetics
  • Enzyme Precursors / metabolism
  • Glycosylation
  • Golgi Apparatus / metabolism*
  • Mutagenesis, Site-Directed
  • Protein Processing, Post-Translational*
  • Proteins / metabolism*
  • Rats
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Transfection

Substances

  • Enzyme Precursors
  • Proteins
  • Recombinant Proteins
  • Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
  • Cathepsin C